منابع مشابه
Improved resolution of asymmetric-PCR SSCP products.
amide gel electrophoresis (PAGE) on a 180× 160× 1.5-mm 10% polyacrylamide gel. The separated DNA strands were subsequently visualized by staining with either EtdBr or silver (2). Separation of the DNA strands before electrophoresis using streptavidin paramagnetic beads consistently yielded single-stranded products upon SSCP analysis (Figure 1). Treatment of DNA strands with S1 nuclease before S...
متن کاملF-SSCP: fluorescence-based polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis.
A fluorescence-based method for polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis, F-SSCP, was developed in which the target sequence is amplified by the PCR using fluorescent primers. The amplified products are then heat-denatured and applied to a water-jacket controlled gel in an automated DNA sequencer. The separated strands are detected as laser-excited f...
متن کاملLigation of 5' DNA overhangs to 3' DNA overhangs using short single-stranded oligonucleotide linkers.
17.Summers, A.O. 1992. Untwist and shout: a heavy metal responsive transcriptional regulator. J. Bacteriol. 174:3097-3101. 18.Trudel, P., S. Provost, B. Massie, P. Chartrand and L. Wall. 1996. pGATA: a positive selection vector based on the toxicity of the transcription factor GATA-1 to bacteria. BioTechniques 20:684-693. 19.Vernet, T., P.C.K. Lau, S.A. Narang and L.P. Visentin. 1985. A direct-...
متن کاملPcr-sscp: a Tool for Molecular Diagnosis of Leptospirosis
Leptospirosis, a disease with protean manifestations, is caused by Leptospira species. Early and reliable identification of Leptospira serovars remains to be a challenge due to diversity at serovar level. The potential of polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) was assessed for detection of sequence variation in pathogenic specific 631 bp fragment of 16S r...
متن کاملArbitrarily primed PCR fingerprints resolved on SSCP gels.
Arbitrarily primed PCR methods (1), including RAPD (2), generate a reproducible fingerprint of DNA products from complex nucleic acids. Differences detected between fingerprints derived from different genomes have been used extensively as polymorphisms in genetic mapping (e.g., 3). Various strategies can be used to maximize the number of polymorphisms that are scored in each fingerprinting expe...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00295bm06